For 2 operations of 16/32 tests of unconcentrated
urinary proteins or for unconcentrated cerebrospinal
fluid. The Silver Blue Stain kit is the only Silver stain
for cellulose acetate existing on the international market and it
is our exclusive. The staining of the bands at the end of the treatment
makes the brown turn into brilliant blue after a bath of few seconds
in the reactive blue toner (included in the kit) giving indelible
conservation of the migrations. The Silver Blue Stain makes it possible
to establish the typical patterns of selective or non-selective
glomerular proteiuria, tubular proteinuria, mixed proteinuria, renal
and post-renal, myelomatose proteinuria of Bence-Jones (K-free or
Lambda-free).
The sensitivity of the method is double that of the Gold Stain methods
and the affinity of the stains to the single proteins in the serum
is sufficiently uniform as demonstrated by the comparative figure.
With simple micro applications of 0.3 µl/4 mm, without wasting
time in costly operations of concentrating of the urine and using
samples from extemporary urination, it is possible to discover myelomatose
proteinuria, K free or Lambda free (Bence-Jones protein), thanks
to the formidable colouring strength of the Silver Blue Stain. The
latter is indelible in time contrary to what happens with the Silver
Stain method on agarose. It must be underlined that the presence
of the Bence-Jones protein in gammapathic patients serves as an
index of malignity of the gammapathy. The patient is immediately
directed to a Haematology and Transplant centre for the necessary
treatment of the case. In the case of unconcentrated cerebrospinal
fluid in micro scale the sample must be deposited 2 or 3 times on
the start point. |