CELLOGEL KITS
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SERUM PROTEINS (100 semimicro or 200 micro tests)
CODE 08C30-R



The kit is intended for the diagnostic clinical electrophoresis of serum proteins for detecting disproteinemias and for quantitating Albumin, Alpha-1, Alpha-2, Transferrin, C3 and Gammaglobulins.


Assessment:
4 semimicro or 8 micro tests per each Cellogel 5.7x14 cm strip.
12 semimicro tests or 24 micro tests per each Cellogel chamber.


Kit content:
Cellogel, Tris-Hippurate buffer, Ponceau S staining, Destaining
solution, Clearing solution, blotting paper and Mylar film
 
 




HIGH RESOLUTION SERUM PROTEINS (150 semimicro or 200 micro tests)
CODE 08C31

Several prestigious authors (Drs. Kohn, Laurell, Aguzzi, Keren et. al.) have not accepted the 20 mm micro electrophoresis of proteins since this technique is not sufficient for diagnosis of gammapathies. HR methods such as Microlong electrophoresis on Cellogel show up to 13 fractions, and have been proposed for diagnosis of incipient gammapathies. In accordance with the Italian Commission for Proteins of SIBioC and some of the most authoritative European experts.

Assessment:
6 semimicro or 8 micro tests per each Cellogel 5.7x14 cm strip.
48 high resolution tests with 6 Cellogel strips placed on 2 Cellogel chamber.

Kit content:
Cellogel, TGS buffer, Coomassie staining, Citric Acid, Clearing solution blotting paper and Mylar film.

Materials not included: Destaining solution (475ml Methanol + 475ml H2O + 50ml Glacial Acetic Acid).

 
 




IFE SERUM + CONCENTRATED URINE (5+5 tests for 5 patients)
CODE 08C09

Simultaneous immunofixation of serum and urine of 1 patient is recommended as unique method for an absolutely certain diagnosis able to observe gammapathies of uncertain significance (MGUS) or the malignancy of the gammapathy, with the presence of a K free or Lambda free monoclonal, or secondary malignancy for evident kidney disease with the presence of an IgG, IgA or IgM monoclonal component in the IFE of serum and urine with relative positivity of alligned K (bound) or Lambda (bound).
This method, proposed in 1984 and appreciated from many SIBioC members, doesn’t use anti K free and anti Lambda free to reveal Bence-Jones protein and respects the guide lines for IFE of the Bence-Jones proposed for urine alone with trivalent anti-serum (anti IgG, anti IgA, anti IgM), anti K Bound & Free and anti Lambda Bound & Free published in Biochimica Clinica, 2001, vol.25, No. 1, pages 23-31.

Assessment:
2 test HRE for each patient in semimicro technique on 6 Cellogel 2.5x14 cm strip placed on 3 bridges in one Cellogel chamber.

Kit content:
Cellogel, TGS buffer, Coomassie staining, Saline solution, Volumetric distributors and Antisera, Clearing solution, blotting paper and Mylar film.

Materials not included: Destaining solution (475ml Methanol + 475ml H2O + 50ml Glacial Acetic Acid).

 
 




IMMUNOFIXATION (24 semimicro or 32 micro tests)
CODE 08C09-2

The kit is intended for the separation and identification of monoclonal gammapathies. When a monoclonal band is revealed by electrophoresis or when an immunoproliferative disorder is suspected, immunofixation of monoclonal components is basic, either to establish true monoclonality of a band, or to establish the nature of the monoclonal component and fix it. In fact different types have different diagnostic and prognostic value.

Assessment:
6 semimicro tests or 8 micro tests on 6 Cellogel 5.7x14 cm strips placed on 6 bridges in two Cellogel chamber.

Kit content:
Cellogel, Tris- Hippurate buffer, Amidoblack staining, Saline solution, Volumetric distributors and Antisera, Clearing solution, blotting paper and Mylar film.

Materials not included: Destaining solution (475ml Methanol + 475ml H2O + 50ml Glacial Acetic Acid).

 
 




HEMOGLOBINS (100 semimicro tests)
CODE 08C35

Electrophoresis of Hemoglobins is a simple laboratory technique for the rapid and accurate detection of abnormal conditions, called hemoglobinopathies. It can reveal the possible existence of hemoglobinopathies in two ways, qualitatively, by indicating the presence or absence of variant hemoglobins, and quantitatively, by making possible the measurement of hemoglobins by densitometry.
The electrophoretic separation of hemoglobins is based on the electrical characteristic of the globin molecule which can be negatively or positively charged depending on the amino acid sequence or composition of the polypeptide chains. Differences in the electrostatic charge will produce differences in electrophoretic mobilities and, hence, separation of the various hemoglobins.

Assessment:
4 semimicro per each Cellogel 5.7x14 cm strip.
12 semimicro tests per each Cellogel chamber.

Kit content:
Cellogel, Tris-Glycine buffer, Ponceau S staining, Destaining solution, Clearing solution, blotting paper, Mylar film and 1 mini box.

 
 




 
GLYCOSYLATED HEMOGLOBINS HbA1c (100 semimicro tests)
CODE 08C64

According to a publication of J. Ambler et al., the non-glycosilated part of Hemoglobin in citrate buffer pH 6.4 containing dextrane sulphate acquires a mobility such as to allow a perfect separation of the glycosilated part. This occurs as the sulphate groups of dextrane combine with non-glycosilated hemoglobin.

Assessment:
4 semimicro per each Cellogel 5.7x14 cm strip.
12 semimicro tests per each Cellogel chamber.

Kit content:
Cellogel, Affinity buffer pH 6.4, Hemolysing solution, Ponceau S staining, Destaining solution, Clearing solution, blotting paper, Mylar film and 1 mini box.

 




LIPOPROTEINS (100 semimicro tests)
CODE 08C42

The Kit is intended for clinical electrophoresis of serum Lipoproteins and evaluation of HDL (Alpha lipo), VLDL (pre ß lipo), LDL (ß lipo) and Chylomicrons fractions.
Hyperlipoproteinemias may be categorized into 5 types according to Fredrickson et Al. by simple observation of electrophoretic pattern, serum appearance and determination of values of Cholesterol and Tryglyceride.
Cellogel is widely used in the world for Lipoproteins testing. More than 20 scientific works have been published on international magazines. Main advantage of Cellogel versus dry Cellulose Acetate or Agarose is the right porosity (Chylomicrons can not penetrate or permeate Cellogel membrane), the suitable thickness of 250-300 microns and combination of both hydrophobic and hydrophilic properties of gelatinized cellulose acetate.

Assessment:
4 semimicro per each Cellogel 5.7x14 cm strip.
12 semimicro tests per each Cellogel chamber.

Kit content:
Cellogel, Tris Hippurate buffer, Sudan Black staining, Clearing solution, blotting paper, Mylar film and 1 mini box.