CELLOGEL ELECTROPHORESIS

In 1997 densitometry of electrophoretic migrations, on strips and sheets of Cellogel, on cellulose acetate supported or not, and also on agarose film, made an extraordinary step forward with the introduction of the first Glob-Al Scan; the electro-mechanical densitometers were capable of reading 8 micro electrophoresis, 4 semi-micro, 16 semi-micro simultaneously on sheets (Multipolar) or one single micro or semi-micro migration on small strips. All these densitometers have been replaced by software technology, scanners, readers of optical density of micro, semi-micro and macro electrophoresis arranged on the normal strips or even on sheets containing up to 144 micro migrations. Glob-Al Scan software, installed on the client’s computer with Windows 98, 2000 or XP, and printer and scanner suggested by us, is capable of reading all types and formats of electrophoresis which fit onto the scanner surface in one single scan. Placing the frames relative to one’s own electrophoretic programme one obtains: a reading of the optical density of all the migrations, densitometric curve of each single migration, the quantative data in absolute values and in percentages of the protein fractions, the ratio albumin globulin and the total protein value. The Glob-Al Scan reads migrations in different colours (e.g. Red, Blue or Green) and regulates filtration so as to obtain a reading of the maximum optic density (maximum absorption); once set on one’s own programme, Glob-Al Scan reproduces the same conditions for quick reading of successive electrophoretic migrations.
The reading of optical density is obtained with the scanner regulated at 72 dpi, value equivalent to photocells of classical densitometers for electrophoresis. The programme performs a complex calculation which mathematically transforms the data of reflection and reflectivity into optical absorption expressed in the usual logarithmic scale from 0 to infinity O.D.