EQUIPMENT
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  POWER SUPPLY
 
Code 10A08/B
The Power Pack 300V is suited to all types of electrophoresis requiring up to 300 V. The digital display indicates the voltage values (which can be constantly regulated) and the current. With two outputs 2 tanks can be supplied at the same time.

Voltage:
0-300 V DC current
Amperage: 0-100 mA
Exit Points: 2 (for 2 tanks)
Timer: 3s - 60h
Input: 220V (on request 110 V) 50/60 Hz
Dimensions: 27x21x11 cm
Weight: 3.8 Kg
equipment and accessories for Cellogel electrophoresis




  ELECTROPHORETIC TANK FOR CELLOGEL
 
Code 11A11-CE
Tank for electrophoresis on Cellogel and cellulose acetate in general. Designed for routine and research needs. Functions with six strips 2.5x14 cm or with three strips 5.7x14 cm on three bridges, model France of 8.5 cm, furthermore it works with the same size strips as above and with sheets 14x14 cm or 18.3x14 cm on an 8.5 cm long bridge with plastic clips. The tank and the bridges are injection moulded in polycarbonate with high chemical and physical resistance. The lid is in smoky grey semi-transparent polycarbonate with two magnets which work safety micro-switches and cut off the current when the lid is taken off.

Electrodes: Platinum, 7 cm
Dimensions: base 25x19 cm, height 5 cm
Capacity: 200 ml of buffer soln. per compartment
Weight (including the 4 bridges): 1 Kg
electrophoretic tank for cellogel




  BRIDGES FOR CELLOGEL TANK

a - Cod. 11B06-1 France bridge, 8.5 cm, for strips of 2.5x14 or 5.7x14 cm.

b - Cod. 11B03 Long bridge for sheets of 18.3x14 or 14x14 cm and strips of 2.5x14 or 5.7x14 cm.Migration field 8.5 cm.

c - Cod. 11B15-1 France bridge, 11 cm, for strips of 2.5x17 or 5.7x17 cm, Rectangular.

d - Cod. 11B04 Long bridge for sheets of 17x17 cm and strips of 5.7x17 cm. Migration field 11 cm.

e - Cod. 11B14 France bridge, 14 cm, for Cellogel RS Wedge of 5x18.5 and 5.7x18.5 cm Rectangular.

equipment and accessories for Cellogel electrophoresis




  APPLICATORS FOR CELLOGEL
   
Micro 8P2 code 12A08/8P2
 
equipment and accessories for Cellogel electrophoresis

equipment and accessories for Cellogel electrophoresis
Figure 1
Can be clipped onto all France bridges. It applies 8 specimens simultaneously to the Cellogel strip of 5.7 cm width, leaving 8 marks of the same dimensions as Figure 1a. Each of the 8 tips applies 0.3 µl on 5 mm. The unloading of the tips is complete after the application time of at least 10 seconds. The specimen holder base has 3 rows of 8 drop holders onto which volumes of 20 µl serum are pipetted. This applicator enables the rapid application of 24 samples on 3 strips already positioned in the chamber. If the first notch is used the deposits are aligned at 27 mm distance from the cathodic edge. If the last notch is used the deposits are aligned 9 mm from the cathodic edge.
The applicator serves not only for classical micro electrophoresis with 20 mm migration but also for the “Microlong”, i.e. high resolution micro-electrophoresis. This latter method is recommendable for the rapid search for monoclonal gammapathies including incipient ones.

Semimicro 2+2 code 12A02/SU

Studied for simultaneous IFE of serum and urine (kit Code 08C09). It can be clipped onto France bridges. It applies 4 specimens simultaneously on two strips, 2.5x14 cm, positioned at the lateral extremities of the bridge. Each of the 4 tips applies 0.7 µl on 7 mm. The unloading of the tips is complete after an application time of at least 10 seconds. For this type of use 25 µl of serum and 25 µl of concentrated urine are pipetted onto the drop holder of the base of the sample holder. It can be utilised for semi-micro electrophoresis of 2 samples on strips 2.5x14 or 2.5x17 cm.
   
Semimicro 4P4 code 12A08/4P4

It can be clipped on all France bridges. It applies 4 specimens simultaneously on the 5.7 cm wide Cellogel strip leaving 4 marks of the same dimensions as the Figure 1c. Each of the 4 tips applies 0.9 µl on 9 mm. The unloading of the tips is completed after an application time of at least 10 seconds. The base of the specimen holder has 3 rows of 4 drop holders onto which 30 µl are pipetted. This applicator makes it possible to deposit 12 samples rapidly on three strips already positioned in the tank. If the first notch is used the deposits will be aligned at 27 cm from the cathodic edge. If the last notch is selected the deposits will be aligned 9 mm from the cathodic edge. This applicator serves not only for classical semi-micro electrophoresis with 35 mm run but also for the prolonged semi-micro of 45-55 mm.
   
Semimicro 4P code 12A05

Recommended for electrophoresis of hemoglobins. It can be clipped onto all France bridges and applies 4 specimens simultaneously onto the 5.7 cm wide Cellogel strips leaving marks of the same dimensions as Figure 1d. Each of the tips applies 1.2 µl on 9 mm. The unloading of the tips is completed after an application time of at least 10 seconds. The base of the specimen holder has 3 rows of 4 drop holders onto which 30 µl serum are pipetted. This applicator renders it possible to deposit 12 specimens rapidly on three strips already positioned in the tank. If the first notch on the bridge is selected, the deposits will be aligned at 27 mm from the cathodic edge. If the last notch is selected the deposits will be aligned at 9 mm from the cathodic edge.
This applicator is utilised with Cellogel 250 µ or more thick, for deposits, even repeated, of serum to determine the lipoproteins or the isoenzymes (e.g. for the gamma GT which needs 4 repeated deposits for a total of about 5 µl). This applicator cannot be used on dry micro-porous acetate membranes because the deposit would spread giving an unacceptable start point for good electrophoresis. The deposit, even repeated, on Cellogel maintains a perfect mark and is a suitable start point for excellent migration.
 
semicro 6p2
Semimicro 6P2 code 12A08/6P2

It can be clipped onto all France bridges and applies 6 specimens simultaneously on the 5.7 cm wide Cellogel strip leaving 6 marks of the same dimensions as Figure 1b. Each of the 6 tips applies 0.7 µl on 7 mm. The unloading of the tips is completed after an application time of at least 10 seconds. The base of the specimen holder has 3 rows of 6 drop holders onto which 25 µl serum are pipetted. This applicator renders it possible to deposit 18 samples rapidly on three strips already positioned in the tank. If the first notch of the bridge is selected the deposits will be aligned at 27 mm from the cathodic edge. If the last notch is selected the deposits will be aligned at 9 mm from the cathodic edge.
   
semicro 18p2
Semimicro 18P2 code 12A20/18P2

It can be clipped onto long bridges (code 11B06 and code 11B15). It applies 18 specimens simultaneously on Cellogel sheets positioned on the above mentioned bridges. Each of the 18 tips applies 0.7 µl on 7 mm. The unloading of the tips is completed after an application time of at least 10 seconds. The disposable specimen holder is moulded in transparent polystyrene and is inserted into the base of the applicator. It holds 3 rows of 18 rectangular sample points, 9 mm pitch, suitable for multiple pipetting dispensing 25 µl of specimens. This applicator can be used for high resolution electrophoresis of 18-36 samples with two tanks.
   
applicator box
Applicator Box, micro, semi-micro and macro, code 12A07
This set is suitable for single applications.
The box contains:
- 1 Applicator micro 0.3 µl/5 mm
- 1 Applicator semi-micro 1.2 µl/9 mm
- 1 Applicator macro 2 µl/15 mm
- 1 Serum holder base for 10 micro-semimicro and 6 macro
- 1 Applicator holder with a lever to lower the tip until contact with the surface of Cellogel.




  ACCESSORIES

 

guide ruler for applicator box
Guide ruler for Applicator Box, code 11B11

This accessory of the applicator box can be placed on the edge of the tank so that the distance between the various deposits can be selected.
   
guide ruler for applicator box
Guide ruler for Applicator Box, code 11B13

This is an important accessory for the applicator box. The ruler can be fastened onto the bridges, codes 11B03 and 11B04.
   
volumetric distributor

Volumetric Distributor, Model DC/3, code 13A23

With this device, moulded in transparent polystyrene, it is possible to distribute on the surface of Cellogel strips, 2.5 cm wide, laid on the electrophoretic bridge, measured volumes of 40, 75 and 100 µl of anti-serum (immunofixation) or an enzymatic substrate (isoenzymes LDH, CPK,Gamma GT, etc) obtaining a large saving in reagents which generally have high costs. Particularly, in the case of the IFE of 1 patient on a semi-micro scale after electrophoresis of serum+urine on 6 strips (Kit code 08C09), the first strip is placed in staining while the second is stratified with the DC/3 Volumetric Distributor with 40 µl of anti IgG anti-serum, 40 mm long in the migration zone. The same is undertaken with the 3rd, 4th, 5th and 6th strips respectively with anti-IgA, anti-IgM, anti-K and anti-Lambda antisera. The five distributors are washed while the 5 strips are left in incubation at room temperature for 15 minutes. At the end of incubation, the strips are washed in saline solution and they are stained with amidoblack (see instructions for use of Kit, code 08C09). Another important use of the Volumetric Distributor is the stratification of the stain substrate to reveal, for example, the LDH isoenzymes of the serum, immediately after the electrophoretic migration of the samples. In this case are sufficient 75 µl of the expensive reagent of NAD, MTT and Fenazine Metasulfate stratified on the surface of 2.5x14 cm strips still laid on the bridge to reveal the 5 isoenzymatic fractions of LDH after 30 minutes incubation at 37°C (see instructions for use of staining solution, code 08C01).
Volumetric Distributor, Model DC/6, code 13A24

This device is used to distribute volumes of 100-150 µl of antisera or enzymatic chromogens on 5.7x14 cm strips laid on the France bridge of the electrophoretic chamber. The distributor DC/6 is ideal for preparing the Cellogel strip impregnated with 2-4 µl/cm2 of specific anti-serum to determine the quantity of single proteins by the Laurell technique (Rockets). See Electroimmunodiffusion instructions for determination of albumin, alfa1 anti-trypsin, anti-Lpa etc. The DC/6 model is also used for two dimensional immunoelectrophoresis to stratify, for example, 140 µl of total anti human antiserum to perform the second dimension consisting of the migration in antibody field of all the human proteins separated during electrophoresis of the first dimension.
 
Plastic Guide for two-dimensional Immunoelectrophoresis, code 13A26

The guide is clipped onto the 8.5 cm bridge on which the 14x14 cm sheet of Cellogel has been placed and is used to deposit the serum diluted 1:4 of the first dimension and to guide the DC/6 distributor in the second dimension at the moment of applying the antiserum. Cellogel is unique for the performance of this technique which is also used by producers of antisera to control their specificity. 2D-IEP on Cellogel offers a higher sensitivity to Agarose and exceptional simplicity because the sample containing immunoglobulins does not retromigrate and does not need preliminary carbamilation to chemically modify the immunoglobins as required by Agarose.
   
Plastic Guide for Crossover, code 13A29

It can be clipped onto France bridges. This guide, moulded in transparent polystyrene, permits the depositing of sera, anti-sera or pure antigens in the Crossover technique or counterimmunoelectrophoresis on buffered Cellogel 5.7x14 cm placed on the bridge.
   
Guide for quantitative Radial Immunodiffusion (Mancini Technique)

This guide is needed only for depositing specimens on “Cellogel/Myl” 6x7.6 cm (Cellogel on a film of Mylar) and impregnated with 1-2 µl of antiserum per cm2. To perform this quantitative technique at very low cost it is necessary to master the use of Microcaps of 1 µl required for a perfect deposit of the samples, generally diluted and pre-stained with Bromophenol Blue.

 
Transparent box

Plastic box for buffering, staining, destaining, washing, etc. processes.

a. Small Box, code 13A53 Dimensions: 22x6x5 cm

b. Minibox, code 13A51Dimensions: 13x7.8x2.5 cm

c. Long Box, code 13A52 Dimensions: 27.8x9.4x5.5 cm

d. Medium Box, code 13A50 Dimensions: 22x11x5.5 cm
 
Mini Transilluminator, code 13A16
The Transilluminator is used for optical intensification of the fractions on dried and whitened Cellogel, the revelation of proteic components up to 20 ng/band and photographic recording of the migrations.

Rotating shaker, code 13A34

This apparatus, simple and strongly built is intended for buffering, staining, destaining, washing in saline solution, etc. The shaker functions at the speed of 60 rpm and is particularly suited to and indispensable for electrophoresis and immunotechniques on Cellogel, such as IFE, Laurell technique, two-dimensional immunoelectrophoresis, etc.

Mylar Film, code 13M01-100

Mylar films, 5.7x10 cm, are recommended in place of glass slides as a support for transparency of Cellogel. This, if the clearing solution (code 06A06-S1) is used, sticks to the support forming a single film. Compared to the procedure on glass, the coupling of Cellogel on Mylar, once extracted from the oven at 80°C, cools in 1 minute and is immediately ready for densitometry.

Mylar Film, code 13M04-100

The Mylar films, 18.3x14 cm, are used to form a sandwich of wet Cellogel with a pattern to photocopy straight after destaining procedure.